Authentication of Averrhoa carambola L. Using DNA Barcoding: Detection of SNPs and Evaluation of Phylogenetic Relationship

Abstract: Background: Averrhoa carambola L. (star fruit) is an underutilized plant in the family Oxalidaceae. The has various pharmacological activities and fleshy juicy fruit with high nutritional value rich vitamins. Objectives: This study aims to authenticate examine evolutionary relationships between taxa. Materials Methods: species identification was verified using well-established DNA barcoding method. For this, a few predetermined loci including rpoB, matK, atpF-atpH, rbcL were employed. Results: Amplicons observed molecular weight of 450bp, 950bp, 800bp, 650bp respectively as reported earlier. sequences these submitted NCBI. approved accession numbers MN447319, MN733917, MT195537, MT195538 respectively. amplified related taxa genus compared bioinformatics tools. 204 SNPs/indels identified out which some are unique for species. K2P analysis showed that maximum intraspecific distance (1.3%) lower than minimum interspecific (2.6%). Conclusion: Among different universal loci, matK found be most informative terms occurrence SNPs genetic distance. So, information can utilized authentication Keywords: carambola, Barcoding, atpF–atpH, rpoB.